Protein_Domain
miniA

Part:BBa_K877000

Designed by: Alie Abele, David Pohlman   Group: iGEM12_Missouri_Miners   (2012-09-29)


Mini CipA coding region

This part codes for a portion of the scaffoldin protein (cipA) from Clostridium thermocellum cellulosome. Two regions of cipA were ligated together to form this part: three type one cohesin regions and a type two dockerin region. A signaling peptide for export of the protein out of the cell is also coded in this part.

This part is unfinished; it contains 6 pst1 sites within its coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 407
    Illegal PstI site found at 1218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 407
    Illegal PstI site found at 1218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 407
    Illegal PstI site found at 1218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 407
    Illegal PstI site found at 1218
    Illegal AgeI site found at 1072
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None